Vittorio Gentile
University of Campania Luigi Vanvitelli, Italy
Title: Neuronutraceuticals modulate transglutaminase 2 over expression as a marker of neuro inflamation in mouse and human cells
Biography
Biography: Vittorio Gentile
Abstract
Background: Tissue type 2 Transglutaminase (TG2, E.C. 2.3.2,13) is reported to be involved in phagocytosis of apoptotic cells in mouse microglial BV2 cells and peripheral macrophages.
In this study, by using Lipopolysaccharide (LPS)- or Amyloid-1-42 (A1-42) peptide-stimulated mouse microglial cells BV2 or human THP-1 cells, we examined the effects of different neuronutraceutical compounds, such as Curcumin (Cu) and NPalmitoylethanolamine (PEA), known for their anti-inflammatory activity, on TG2 and several inflammatory or neuroprotective biomarkers expressions.
Methods: Mouse BV2 or human THP-1 cells were treated with LPS or Aβ1-42 in presence of Curcumin or PEA, in order to evaluate the expression of TG2 and other inflammatory or neuroprotective markers by RealTime PCR and Western Blot analyses.
Results: Curcumin and PEA were capable to reduce TG2 expression in mouse BV2 or human THP-1 cells during co-treatment with LPS or A1-42
Conclusions: The results show a role for TG2 as an important marker of neuroinflammation and suggest a possible use of Curcumin and PEA, in order to reduce LPS- or Aï¢1-42-induced T2 overexpression in mouse BV2 or human THP-1 cells.